Abstract
Italy
was one of the first country where Gambusia species was introduced
as mosquito control agent at the start of last century. This species is
here identified by the structure of the males gonopodium.
Introduction
Almost
45 valid species belong to the genus Gambusia (Page
et al.1991; Lydeard et al.1995; Lucinda 2003) and are widely spread in
North, Central and South
America, Caribean islands included.
Two of these species,
specifically Gambusia
affinis (Baird & Girard 1853) and Gambusia holbrooki (Girard
1859) were widely introduced through out the whole world for mosquito
population control purposes during the early 20th century.
The systematic
position of both species was clarified in Etnier et al., 1993 (see also
Wooten et al. 1998).
Italy was one of the
first countries where Gambusia has been introduced as mosquito
control agent in 1925 (see Stapleton 2004). Until the Seventies (1970),
their presence was mainly registered in Northern Italy and along the
Tirrenic shore, but during recent years, many new populations arose also
along the east coast and in the South.
For the same reasons,
specimens have been introduced also in Spain, France, Ex-Jugoslavia,
Albania, Greece and as far as the Balcanic area, Russia included (see
Grimaldi & Manzoni 1990) and successively all over the world.
Many articles and
books report the presence of both this species, but for sometime only one
is considered alive in our country, but they usually do not report any
specific study or test to confirm that. For this reason and also due to
our interest in the Italian killifish, Aphanius fasciatus (Valenciennes
in Humboldt & Valenciennes 1821), that often share the same body of
water with Gambusia, we decided to work on this topic.
The purpose of the present study is to clarify that situation,
identifying the species introduced in Italy by the structure of the males
gonopodium.
Material
and Methods
Male
specimens of 4 different populations, from all the
Italian territory (north, south and Islands), was collected with
the help of hand dip net and then preserved in alcohol.
The
localities of collections were:
Modena (Sant
Anna, June 2004, 44°36’25” N 11°01’05”E ),
Palermo (Giardino
zoologico, April 2005, 38°06’56”N 13°21’40”E),
Lecce (ponds near University, May 2005, 40°21’12”N 18°10’26”E)
Cagliari ( collected on February 2006, Lago Poggio dei Pini,
Capoterra ,
39° 08' 48" N 8° 58' 29"E).
In
order to examine the gonopodium, and take a photograph of it, each
specimen was taken from the test tube containing the alcoholic solution
and then washed with pure water.
The gonopodium of
each male, has been removed surgically using a bisturi and pliers for
dissection, then it was put in to a little observation chamber that
allowed examination through an optical microscope.
The tissues were lit
by a white-light lamp. The kind of image we were looking for, was obtained
both using 10x and 20x objective lenses and then it was snapped with a
digital camera, connected to the microscope, and then saved on the PC.
The identification of
the species is based on the differences of the males gonopodium structure
(see Etnier et al. 1993).
Discussion
All
the specimens we tested, by taking photographs of their gonopodium, show
clearly that the distal segments of the most ventral element of the
gonopodium, is finely serrate dorsally.
Thus, even if many
authors, indicate that G. affinis is the mainly widespread mosquito
fish in our country, this study is definitely a clear indication that the
species introduced, and still living, in Italy is G. holbrooki.
The gonopodium
structure is frequently used to discriminate species see as eg. Meyer&Schartl
(2002), Poeser (2002) and Chambers (1987).
We hope that this
work may interest more people, in order to get more material and
information over this topic.
Acknowledgements
We
want to thank Fracesco Denitto, Federico Marrone, Michele Silvestri and
Alessandro Spiga for the preserved specimens. Thanks to Daniele Cavazzoni
for assistance in preparing the first draft of the manuscript
Thanks
to Alessandro Tozzi and Alessandro Spiga for the pictures .
We
thank the Animal Biology and Ecology department of the Università degli
Studi di Cagliari, in particular Dr. Marisa Gaviano, who allowed us to
work with microscopes for this research.
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